NBP2-15776

antibody from Novus Biologicals

Targeting: IL6ST CD130, GP130, sGP130

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP2-15776

Provider

Novus Biologicals

Product name

Rabbit Polyclonal gp130/CD130 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified.

Reactivity

Human, Rat

Host

Rabbit

Isotype

IgG

Vial size

0.1 ml

Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: gp130/CD130 Antibody [NBP2-15776] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with CD130 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: gp130/CD130 Antibody [NBP2-15776] - J5 xenograft, using CD130 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: gp130/CD130 Antibody [NBP2-15776] - Human breast carcinoma. CD130 stained by CD130 antibody diluted at 1:7500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.