MAB5029

antibody from Novus Biologicals

Targeting: IL6ST CD130, GP130, sGP130

Western blot (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB5029

Provider

Novus Biologicals

Product name

Mouse Monoclonal gp130/CD130 Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Detects rat gp130 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human or recombinant mouse gp130 is observed.

Reactivity

Rat

Host

Mouse

Conjugate

Unconjugated

Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Rat gp130 by Western Blot. Western blot shows lysates of NRK rat normal kidney cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Rat gp130 Monoclonal Antibody (Catalog # MAB5029) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for gp130 at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Flow cytometry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of gp130 in CD3+ Rat Splenocytes by Flow Cytometry. CD3+ rat splenocytes were stained with Mouse Anti-Rat gp130 Monoclonal Antibody (Catalog # MAB5029, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).