Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Blocking/Neutralizing [1]
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- Product number
- MAB4682-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse gp130 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects mouse gp130 ELISAs. In sandwich immunoassays, no cross-reactivity or interference with recombinant human gp130, recombinant mouse (rm) rmIL-6, or rmIL-6R is observed.
- Reactivity
- Mouse
- Host
- Rat
- Conjugate
- Unconjugated
- Antigen sequence
Q00560
- Isotype
- IgG
- Antibody clone number
- 125605
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Frontline Science: Proliferation of Ly6C+ monocytes during urinary tract infections is regulated by IL-6 trans-signaling.
Rapid monocyte infiltration following retinal detachment is dependent on non-canonical IL6 signaling through gp130.
Microarray and proteomic analysis of breast cancer cell and osteoblast co-cultures: role of osteoblast matrix metalloproteinase (MMP)-13 in bone metastasis.
Dixit A, Bottek J, Beerlage AL, Schuettpelz J, Thiebes S, Brenzel A, Garbers C, Rose-John S, Mittrücker HW, Squire A, Engel DR
Journal of leukocyte biology 2018 Jan;103(1):13-22
Journal of leukocyte biology 2018 Jan;103(1):13-22
Rapid monocyte infiltration following retinal detachment is dependent on non-canonical IL6 signaling through gp130.
Wang X, Miller EB, Goswami M, Zhang P, Ronning KE, Karlen SJ, Zawadzki RJ, Pugh EN Jr, Burns ME
Journal of neuroinflammation 2017 Jun 23;14(1):121
Journal of neuroinflammation 2017 Jun 23;14(1):121
Microarray and proteomic analysis of breast cancer cell and osteoblast co-cultures: role of osteoblast matrix metalloproteinase (MMP)-13 in bone metastasis.
Morrison C, Mancini S, Cipollone J, Kappelhoff R, Roskelley C, Overall C
The Journal of biological chemistry 2011 Sep 30;286(39):34271-85
The Journal of biological chemistry 2011 Sep 30;286(39):34271-85
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Supportive validation
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- R&D Systems (provider)
- Main image
- Experimental details
- Cell Proliferation Induced by Human IL-6 and Neutralization by Mouse gp130 Antibody. Recombinant Human IL-6 (Catalog # 206-IL) stimulates proliferation in the 7TD1 mouse hybridoma line. (orange line). Proliferation elicited by Recombinant Human IL-6 (25 pg/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse gp130 Monoclonal Antibody (Catalog # MAB4682). The ND50 is typically 0.15-1 µg/mL.