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Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Blocking/Neutralizing [1]
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- Product number
- MAB628-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human gp130 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from ascites. Detects human gp130 in ELISAs. In sandwich immunoassays, no significant cross-reactivity or interference with recombinant human (rh) CNTF, rhIL-6, rhIL-6 R, recombinant mouse (rm) IL-6, rhIL-11, rhLIF, rhLIF R, rmLIF, or rhOSM is observed. This antibody blocks the human gp130-mediated bioactivities induced by IL-6, IL-11, LIF, OSM, and CNTF.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
P40189- Isotype
- IgG
- Antibody clone number
- 28105
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Immunocytochemical detection and RT-PCR expression of leukaemia inhibitory factor and its receptor in human fetal and adult ovaries.
Abir R, Fisch B, Jin S, Barnnet M, Freimann S, Van den Hurk R, Feldberg D, Nitke S, Krissi H, Ao A
Molecular human reproduction 2004 May;10(5):313-9
Molecular human reproduction 2004 May;10(5):313-9
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Cell Proliferation Induced by Oncostatin M/OSM and Neutralization by Human gp130 Antibody. Recombinant Human Oncostatin M/OSM stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Human Oncostatin M/OSM (0.8 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human gp130 Monoclonal Antibody (Catalog # MAB628). The ND50 is typically 0.02-0.1 µg/mL.
