Antibody data
- Antibody Data
- Antigen structure
- References [7]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- MAB4681 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse gp130 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects mouse gp130 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human gp130 is observed.
- Reactivity
- Mouse
- Host
- Rat
- Conjugate
- Unconjugated
- Antigen sequence
Q6PDI9
- Isotype
- IgG
- Antibody clone number
- 125623
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Granulocytes Are Unresponsive to IL-6 Due to an Absence of gp130.
Dendritic cell MST1 inhibits Th17 differentiation.
Epstein-Barr virus-induced gene 3 (EBI3) can mediate IL-6 trans-signaling.
Oncostatin M acting via OSMR, augments the actions of IL-1 and TNF in synovial fibroblasts.
Ifitm3 limits the severity of acute influenza in mice.
Essential roles of IL-6 trans-signaling in colonic epithelial cells, induced by the IL-6/soluble-IL-6 receptor derived from lamina propria macrophages, on the development of colitis-associated premalignant cancer in a murine model.
TH and NPY in sympathetic neurovascular cultures: role of LIF and NT-3.
Wilkinson AN, Gartlan KH, Kelly G, Samson LD, Olver SD, Avery J, Zomerdijk N, Tey SK, Lee JS, Vuckovic S, Hill GR
Journal of immunology (Baltimore, Md. : 1950) 2018 May 15;200(10):3547-3555
Journal of immunology (Baltimore, Md. : 1950) 2018 May 15;200(10):3547-3555
Dendritic cell MST1 inhibits Th17 differentiation.
Li C, Bi Y, Li Y, Yang H, Yu Q, Wang J, Wang Y, Su H, Jia A, Hu Y, Han L, Zhang J, Li S, Tao W, Liu G
Nature communications 2017 Feb 1;8:14275
Nature communications 2017 Feb 1;8:14275
Epstein-Barr virus-induced gene 3 (EBI3) can mediate IL-6 trans-signaling.
Chehboun S, Labrecque-Carbonneau J, Pasquin S, Meliani Y, Meddah B, Ferlin W, Sharma M, Tormo A, Masson JF, Gauchat JF
The Journal of biological chemistry 2017 Apr 21;292(16):6644-6656
The Journal of biological chemistry 2017 Apr 21;292(16):6644-6656
Oncostatin M acting via OSMR, augments the actions of IL-1 and TNF in synovial fibroblasts.
Le Goff B, Singbrant S, Tonkin BA, Martin TJ, Romas E, Sims NA, Walsh NC
Cytokine 2014 Aug;68(2):101-9
Cytokine 2014 Aug;68(2):101-9
Ifitm3 limits the severity of acute influenza in mice.
Bailey CC, Huang IC, Kam C, Farzan M
PLoS pathogens 2012 Sep;8(9):e1002909
PLoS pathogens 2012 Sep;8(9):e1002909
Essential roles of IL-6 trans-signaling in colonic epithelial cells, induced by the IL-6/soluble-IL-6 receptor derived from lamina propria macrophages, on the development of colitis-associated premalignant cancer in a murine model.
Matsumoto S, Hara T, Mitsuyama K, Yamamoto M, Tsuruta O, Sata M, Scheller J, Rose-John S, Kado S, Takada T
Journal of immunology (Baltimore, Md. : 1950) 2010 Feb 1;184(3):1543-51
Journal of immunology (Baltimore, Md. : 1950) 2010 Feb 1;184(3):1543-51
TH and NPY in sympathetic neurovascular cultures: role of LIF and NT-3.
Damon DH
American journal of physiology. Cell physiology 2008 Jan;294(1):C306-12
American journal of physiology. Cell physiology 2008 Jan;294(1):C306-12
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- gp130 in M1 Mouse Cell Line. gp130 was detected in immersion fixed M1 mouse myeloid leukemia cell line using Rat Anti-Mouse gp130 Monoclonal Antibody (Catalog # MAB4681) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counter-stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of gp130 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse CD3 PE-conjugated Monoclonal Antibody (Catalog # FAB4841P) and either (A) Rat Anti-Mouse gp130 Monoclonal Antibody (Catalog # MAB4681) or (B) Rat IgG2A Isotype Control (Catalog # MAB006) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). View our protocol for Staining Membrane-associated Proteins.