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Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Blocking/Neutralizing [1]
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- Product number
- AF468 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse gp130 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects mouse gp130 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human gp130 is observed.
- Reactivity
- Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
Q6PDI9- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Cachexia-associated adipose loss induced by tumor-secreted leukemia inhibitory factor is counterbalanced by decreased leptin.
Innate Stat3-mediated induction of the antimicrobial protein Reg3γ is required for host defense against MRSA pneumonia.
Role of interleukin-6 in cardiomyocyte/cardiac fibroblast interactions during myocyte hypertrophy and fibroblast proliferation.
Arora GK, Gupta A, Narayanan S, Guo T, Iyengar P, Infante RE
JCI insight 2018 Jul 26;3(14)
JCI insight 2018 Jul 26;3(14)
Innate Stat3-mediated induction of the antimicrobial protein Reg3γ is required for host defense against MRSA pneumonia.
Choi SM, McAleer JP, Zheng M, Pociask DA, Kaplan MH, Qin S, Reinhart TA, Kolls JK
The Journal of experimental medicine 2013 Mar 11;210(3):551-61
The Journal of experimental medicine 2013 Mar 11;210(3):551-61
Role of interleukin-6 in cardiomyocyte/cardiac fibroblast interactions during myocyte hypertrophy and fibroblast proliferation.
Fredj S, Bescond J, Louault C, Delwail A, Lecron JC, Potreau D
Journal of cellular physiology 2005 Aug;204(2):428-36
Journal of cellular physiology 2005 Aug;204(2):428-36
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Supportive validation
- Submitted by
- R&D Systems (provider)
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- Experimental details
- IL-6 Inhibition of Cell Proliferation and Neutralization by Mouse gp130 Antibody. Recombinant Human IL-6 (Catalog # 206-IL) inhibits proliferation in the the M1 mouse myeloid leukemia cell line in a dose-dependent manner (orange line). Activity elicited by Recombinant Human IL-6 (20 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse gp130 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF468). The ND50 is typically 0.3-1.0 µg/mL.
