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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Immunohistochemistry [4]
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- Product number
- MA5-34888 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GP130 Monoclonal Antibody (B11-B9-A8)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- Positive Control: CD130 recombinant protein, Raji cell and mouse lung tissue lysates, HUVEC, LOVO, human prostate tissue, human liver tissue, human kidney tissue, human pancreas tissue.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- B11-B9-A8
- Vial size
- 100 µL
- Concentration
- 2 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CD130 in Raji cell (1) and mouse lung tissue (2) lysates. Samples were incubated with CD130 monoclonal antibody (Product # MA5-34888), at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CD130 in CD130 recombinant protein. Samples were incubated with CD130 monoclonal antibody (Product # MA5-34888), at a dilution of 1:5000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of CD130 in LOVO cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with CD130 monoclonal antibody (Product # MA5-34888), followed by DAPI (blue).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of CD130 in HUVEC cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with CD130 monoclonal antibody (Product # MA5-34888), followed by DAPI (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of CD130 in paraffin-embedded human prostate tissue. Samples were incubated with CD130 monoclonal antibody (Product # MA5-34888), and followed by hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of CD130 in paraffin-embedded human pancreas tissue. Samples were incubated with CD130 monoclonal antibody (Product # MA5-34888), and followed by hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of CD130 in paraffin-embedded human liver tissue. Samples were incubated with CD130 monoclonal antibody (Product # MA5-34888), and followed by hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of CD130 in paraffin-embedded human kidney tissue. Samples were incubated with CD130 monoclonal antibody (Product # MA5-34888), and followed by hematoxylin.
