Explore
Validate
Learn
Western blot
Immunoprecipitation
Other assayAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Other assay [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- A305-476A - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SNX5 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- 4° C
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human SNX5 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SNX5 antibody A305-476A (lot A305-476A-1) used for IP at 6 µg per reaction. SNX5 was also immunoprecipitated by rabbit anti-SNX5 antibody A305-493A. For blotting immunoprecipitated SNX5, A305-476A was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
