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antibody from Novus Biologicals
Targeting: GOLM1
bA379P1.3, C9orf155, FLJ23608, GOLPH2, GP73
Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Immunohistochemistry [1]
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- Product number
- NBP2-27212 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal GOLM1 Antibody
- Antibody type
- Polyclonal
- Description
- Protein G purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Antitumor activity and molecular effects of the novel heat shock protein 90 inhibitor, IPI-504, in pancreatic cancer.
Differential membrane proteomics using 18O-labeling to identify biomarkers for cholangiocarcinoma.
Song D, Chaerkady R, Tan AC, García-García E, Nalli A, Suárez-Gauthier A, López-Ríos F, Zhang XF, Solomon A, Tong J, Read M, Fritz C, Jimeno A, Pandey A, Hidalgo M
Molecular cancer therapeutics 2008 Oct;7(10):3275-84
Molecular cancer therapeutics 2008 Oct;7(10):3275-84
Differential membrane proteomics using 18O-labeling to identify biomarkers for cholangiocarcinoma.
Kristiansen TZ, Harsha HC, Grønborg M, Maitra A, Pandey A
Journal of proteome research 2008 Nov;7(11):4670-7
Journal of proteome research 2008 Nov;7(11):4670-7
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: GOLM1 Antibody [NBP2-27212] - IHC analysis of formalin fixed paraffin-embedded (FFPE) human stomach using GOLM1 antibody at 1:100 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Golgi staining was observed. Staining was performed by Histowiz.
