AF2285a

antibody from Abgent

Targeting: GOLM1 bA379P1.3, C9orf155, FLJ23608, GOLPH2, GP73

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AF2285a

Provider

Abgent

Product name

GOLPH2 Antibody (C-Term)

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Peptide-affinity purified goat antibody

Host

Goat

Conjugate

Unconjugated

Isotype

IgG

Vial size

0.1mg

Storage

Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -70°C in small aliquots to prevent freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human GOLM1 with C-terminal MYC tag probed with AF2285a (1ug/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK293 probed with AF2285a (1mg/ml) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence.

Immunohistochemistry

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

"AF2285a (3.8 µg/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining."