PA5-80668
antibody from Invitrogen Antibodies
Targeting: GOLM1
bA379P1.3, C9orf155, FLJ23608, GOLPH2, GP73
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
- Other assay [1]
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Validation data
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- Product number
- PA5-80668 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GOLPH2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of GOLPH2 in Lane A: Jurkat Whole Cell Lysate (30 µg), Lane B: 293T Whole Cell Lysate (30 µg), Lane C: Hela Whole Cell Lysate (30 µg), Lane D: A549 Whole Cell Lysate (30 µg). Samples were probed using a GOLPH2 Polyclonal Antibody (Product # PA5-80668) at a 1:500 dilution, followed by a Goat Anti-Rabbit IgG (H+L), Dylight 800 Secondary Antibody at a 1:10000 dilution. Western blot was performed under reducing conditions. Predicted band size:45 kDa. Observed band size:72 kDa.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of GOLPH2 in Lane A: GOLM1 konckout Hela Whole Cell Lysate (10 µg), Lane B: Hela Whole Cell Lysate (10 µg). Samples were probed using a GOLPH2 Polyclonal Antibody (Product # PA5-80668) at a 1:500 dilution, followed by a Goat Anti-Rabbit IgG (H+L), HRP Secondary Antibody at a 1:10000 dilution. Western blot was performed under reducing conditions. Predicted band size:45 kDa. Observed band size: kDa.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using GOLPH2 Polyclonal Antibody (Product # PA5-80668) at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate, Lane B: 293T Whole Cell Lysate, Lane C: Hela Whole Cell Lysate, Lane D: A549 Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Rabbit IgG H&L (DyLight™ 800) at 1:10,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 45 kDa. Observed band size: 72 kDa. (We are unsure of the identity of these extra bands).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using GOLPH2 Polyclonal Antibody (Product # PA5-80668) at 1:500 dilution. Lane A: GOLPH2 konckout Hela Whole Cell Lysate, Lane B: Hela Whole Cell Lysate. Lysates/proteins at 10 μg per lane. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1:10,000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 45 kDa. Observed band size: kDa.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence staining of GP73 in HeLa cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with GOLPH2 Polyclonal Antibody (Product # PA5-80668, 1:1,000) at 4°C overnight. Then cells were stained with the Alexa Fluor®594-conjugated Goat Anti-rabbit IgG secondary antibody (red) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of human GOLPH2 in human hepatoma with GOLPH2 Polyclonal Antibody (Product # PA5-80668, 1:5,000, formalin-fixed paraffin embedded sections).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of human GOLPH2 in human liver with GOLPH2 Polyclonal Antibody (Product # PA5-80668, 1:5,000, formalin-fixed paraffin embedded sections).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- GOLPH2 Immunoprecipitation using: Lane A: 0.5 mg Hela Whole Cell Lysate 2 µL with GOLPH2 Polyclonal Antibody (Product # PA5-80668) and 15 µL of 50 % Protein G agarose. Primary antibody: GOLPH2 Polyclonal Antibody, at 1:200 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 45 kDa. Observed band size: 70 kDa.