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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- ELISA [1]
- Immunocytochemistry [1]
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- Product number
- H00010434-M05 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00010434-M05, RRID:AB_1146125
- Product name
- Mouse Monoclonal LYPLA1 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. LYPLA1 - lysophospholipase I (3D5)
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Thioesterase activity and subcellular localization of acylprotein thioesterase 1/lysophospholipase 1.
Hirano T, Kishi M, Sugimoto H, Taguchi R, Obinata H, Ohshima N, Tatei K, Izumi T
Biochimica et biophysica acta 2009 Aug;1791(8):797-805
Biochimica et biophysica acta 2009 Aug;1791(8):797-805
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: LYPLA1 Antibody (3D5) [H00010434-M05] - Analysis of LYPLA1 expression in transfected 293T cell line by LYPLA1 monoclonal antibody (M05), clone 3D5. Lane 1: LYPLA1 transfected lysatE (24.7 KDa). Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: LYPLA1 Antibody (3D5) [H00010434-M05] - Detection limit for recombinant GST tagged LYPLA1 is 0.1 ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: LYPLA1 Antibody (3D5) [H00010434-M05] - Analysis of monoclonal antibody to LYPLA1 on HeLa cell. Antibody concentration 10 ug/ml
