Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Immunocytochemistry [1]
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Validation data
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- Product number
- PA5-31242 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PCBP3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: NT2D1, IMR32, U87-MG, MCF-7, Neuro 2A, C8D30, NIH-3T3, Raw264.7, C2C12, PC-12, Rat-2. Predicted reactivity: Mouse (89%), Rat (88%), Bovine (82%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of PCBP3 was performed by separating 30 µg of various whole cell extracts by 12% SDS-PAGE. Proteins were transferred to a membrane and probed with a PCBP3 Polyclonal Antibody (Product # PA5-31242) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of PCBP3 was performed by separating 30 µg of various whole cell extracts by 12% SDS-PAGE. Proteins were transferred to a membrane and probed with a PCBP3 Polyclonal Antibody (Product # PA5-31242) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using PCBP3 Polyclonal Antibody (Product # PA5-31242). Sample (30 µg of whole cell lysate). Lane A: IMR32. 12% SDS PAGE. PCBP3 Polyclonal Antibody (Product # PA5-31242) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of PCBP3 was performed by separating 30 µg of various whole cell extracts by 12% SDS-PAGE. Proteins were transferred to a membrane and probed with a PCBP3 Polyclonal Antibody (Product # PA5-31242) at a dilution of 1:1000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of PCBP3 in methanol-fixed MCF-7 cells using a PCBP3 polyclonal antibody (Product # PA5-31242) at a 1:500 dilution.