HPA001221

antibody from Atlas Antibodies

Targeting: ESS2 bis1, DGCR13, DGCR14, DGS-H, DGSI, ES2, Es2el, ESS-2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Enhanced validation)

Antibody Data

Product number

HPA001221

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA001221, RRID:AB_1078645

Product name

Anti-DGCR14

Antibody type

Polyclonal

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

ASASSLLLPAASRPPRKREAGEAGAATSKQRVLDE
EEYIEGLQTVIQRDFFPDVEKLQAQKEYLEAEENG
DLERMRQIAIKFGSALGKMSREPPPPYVTPATFET
PEVHAGTGVVGNKPRPRGRGLEDGEAGEEEEKEPL
PSLDV


Isotype

IgG

Vial size

100 µl

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Western blot

Supportive validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Recombinant expression validation

Main image

Experimental details

Western blot analysis in control (vector only transfected HEK293T lysate) and DGCR14 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY411609).

Immunocytochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.

Sample type

HUMAN

Immunohistochemistry

Enhanced validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Independent antibody validation

Main image

Experimental details

Immunohistochemical staining of human cerebral cortex, colon, liver and testis using Anti-DGCR14 antibody HPA001221 (A) shows similar protein distribution across tissues to independent antibody HPA001222 (B).

Antibody #2 product nr

HPA001222

Antibody provider

Atlas Antibodies

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Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human gallbladder shows moderate nuclear positivity in glandular cells.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human testis using Anti-DGCR14 antibody HPA001221.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human cerebral cortex using Anti-DGCR14 antibody HPA001221.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human liver using Anti-DGCR14 antibody HPA001221.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human colon using Anti-DGCR14 antibody HPA001221.

Sample type

HUMAN