Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Flow cytometry [2]
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Validation data
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- Product number
- FAB2365G - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rat Monoclonal TIM-3 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human TIM-3 in direct ELISAs and Western blots. Does not cross-react with recombinant human (rh) TIM-1, rhTIM-4, recombinant mouse (rm) TIM-1, rmTIM-2, rmTIM-3, rmTIM-5, or rmTIM-6.
- Reactivity
- Human
- Host
- Rat
- Conjugate
- Green dye
- Isotype
- IgG
- Vial size
- 100 Tests
- Storage
- Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 degreesC as supplied.
Submitted references Arming of MAIT Cell Cytolytic Antimicrobial Activity Is Induced by IL-7 and Defective in HIV-1 Infection.
Activation, exhaustion, and persistent decline of the antimicrobial MR1-restricted MAIT-cell population in chronic HIV-1 infection.
Leeansyah E, Svärd J, Dias J, Buggert M, Nyström J, Quigley MF, Moll M, Sönnerborg A, Nowak P, Sandberg JK
PLoS pathogens 2015 Aug;11(8):e1005072
PLoS pathogens 2015 Aug;11(8):e1005072
Activation, exhaustion, and persistent decline of the antimicrobial MR1-restricted MAIT-cell population in chronic HIV-1 infection.
Leeansyah E, Ganesh A, Quigley MF, Sönnerborg A, Andersson J, Hunt PW, Somsouk M, Deeks SG, Martin JN, Moll M, Shacklett BL, Sandberg JK
Blood 2013 Feb 14;121(7):1124-35
Blood 2013 Feb 14;121(7):1124-35
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of TIM-3 in Human PBMCs Monocytes by Flow Cytometry. Human peripheral blood mononuclear cell (PBMC) monocytes were stained with Rat Anti-Human TIM-3 Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # FAB2365G, filled histogram) or isotype control antibody (Catalog # IC006G, open histogram). View our protocol for Staining Membrane-associated Proteins.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of TIM-3 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) either (A) untreated or (B) treated with Recombinant Human IL-12 (Catalog # 219-IL) and Goat Anti-Human IL-4 Polyclonal Antibody (Catalog # AB-204-NA) to induce Th1 development for 3 days were stained with Mouse Anti-Human CD4 PE-conjugated Monoclonal Antibody (Catalog # FAB3791P) and either (A) Rat Anti-Human TIM-3 Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # FAB2365G) or (B) Rat IgG2A Alexa Fluor 488 Isotype Control (Catalog # IC006G). Dot plots were gated on lymphocytes. View our protocol for Staining Membrane-associated Proteins.