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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Flow cytometry [1]
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- Product number
- FAB2365T-025 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rat Monoclonal TIM-3 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human TIM-3 in direct ELISAs and Western blots. Does not cross-react with recombinant human (rh) TIM-1, rhTIM-4, recombinant mouse (rm) TIM-1, rmTIM-2, rmTIM-3, rmTIM-5, or rmTIM-6.
- Reactivity
- Human
- Host
- Rat
- Conjugate
- Red dye
- Isotype
- IgG
- Vial size
- 25 Tests
- Storage
- Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 degreesC as supplied.
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Detection of TIM-3 in Th1-stimulated Human PBMCs by Flow Cytometry. Unstimulated (A) and Th1-stimulated (B) human peripheral blood mononuclear cells (PBMCs) were stained with Rat Anti-Human TIM-3 Alexa Fluor® 594-conjugated Monoclonal Antibody (Catalog # FAB2365T) and Mouse Anti-Human CD4 APC-conjugated Monoclonal Antibody (Catalog # FAB3791A). Quadrant markers were set based on control antibody staining (Catalog # IC006T). View our protocol for Staining Membrane-associated Proteins.
