PA5-46989

antibody from Invitrogen Antibodies

Targeting: HAVCR2 CD366, FLJ14428, Tim-3, TIM3, TIMD3

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Recommended by provider)

Antibody Data

Product number

PA5-46989

Provider

Invitrogen Antibodies

Product name

TIM3 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

In direct ELISAs, approximately 5% cross-reactivity with recombinant mouse (rm) TIM-3 is observed and less than 1% cross-reactivity with recombinant human TIM-1, rmTIM-1, and rmTIM-2 is observed. Reconstitute at 0.2 mg/mL in sterile PBS.

Reactivity

Human

Host

Goat

Isotype

IgG

Vial size

100 µg

Concentration

0.2 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Knockout validation by Western blot analysis of TIM-3 in lysates of HDLM-2 human Hodgkin’s lymphoma cell line and human TIM-3 knockout HDLM-2 human Hodgkin’s lymphoma cell line (KO). Samples were incubated in TIM-3 polyclonal antibody (Product # PA5-46989) using a dilution of 1 µg/mL followed by a HRP-conjugated Anti-Goat IgG secondary antibody. A specific band was detected for TIM-3 at approximately 50 kDa (as indicated) in the parental HDLM-2 human Hodgkin’s lymphoma cell line, but is not detectable in knockout HDLM-2 human Hodgkin’s lymphoma cell line. GAPDH is shown as a loading control. This experiment was conducted under reducing conditions.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of TIM3 in immersion fixed paraffin-embedded sections of human tonsil. Samples were incubated with TIM3 polyclonal antibody (Product # PA5-46989) using a dilution of 3 µg/mL for 1 hour at room temperature followed by Anti-Goat IgG VisUCyte™ HRP Polymer Antibody. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic. Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell membranes and extracellular space.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of TIM3 in formalin-fixed paraffin-embedded tissue sections of human tonsil probed with ACD RNAScope® Probe and stained using ACD RNAscope® 2.5 HD Detection Reagents-Red (top image). Samples were incubated in TIM3 polyclonal antibody (Product # PA5-46989) using a dilution of 3 µg/mL for 1 hour at room temperature followed by Anti-Mouse IgG VisUCyte HRP Polymer Antibody and DAB chromogen (lower image, yellow-brown). Tissues were counterstained with hematoxylin (blue).