Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Flow cytometry [1]
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Validation data
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- Product number
- FAB2365N - Provider product page
- Provider
- R&D Systems
- Product name
- Human TIM-3 Alexa Fluor® 700-conjugated Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human TIM-3 in direct ELISAs and Western blots. Does not cross-react with recombinant human (rh) TIM-1, rhTIM-4, recombinant mouse (rm) TIM-1, rmTIM-2, rmTIM-3, rmTIM-5, or rmTIM-6.
- Reactivity
- Human
- Host
- Rat
- Conjugate
- Near infrared dye
- Antigen sequence
Q8TDQ0.2
- Isotype
- IgG
- Antibody clone number
- 344823
- Vial size
- 100 Tests
- Storage
- Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied.
Submitted references Effect of IL-7 Therapy on Phospho-Ribosomal Protein S6 and TRAF1 Expression in HIV-Specific CD8 T Cells in Patients Receiving Antiretroviral Therapy.
Increased frequency of Tim-3 expressing T cells is associated with symptomatic West Nile virus infection.
Wang C, Edilova MI, Wagar LE, Mujib S, Singer M, Bernard NF, Croughs T, Lederman MM, Sereti I, Fischl MA, Kremmer E, Ostrowski M, Routy JP, Watts TH
Journal of immunology (Baltimore, Md. : 1950) 2018 Jan 15;200(2):558-564
Journal of immunology (Baltimore, Md. : 1950) 2018 Jan 15;200(2):558-564
Increased frequency of Tim-3 expressing T cells is associated with symptomatic West Nile virus infection.
Lanteri MC, Diamond MS, Law JP, Chew GM, Wu S, Inglis HC, Wong D, Busch MP, Norris PJ, Ndhlovu LC
PloS one 2014;9(3):e92134
PloS one 2014;9(3):e92134
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Supportive validation
- Submitted by
- R&D Systems (provider)
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- Experimental details
- Detection of TIM-3 in Human PBMCs by Flow Cytometry. Human peripheral blood mono-nuclear cells (PBMCs) were stained with Rat Anti-Human TIM-3 Alexa Fluor® 700-conjugated Mono-clonal Antibody (Catalog # FAB2365N) and Mouse Anti-Human CD14 PE-conjugated Mono-clonal Antibody (Catalog # FAB3832P). Quadrant markers were set based on control antibody staining (Catalog # IC006N). View our protocol for Staining Membrane-associated Proteins.