Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- H00023597-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00023597-M01, RRID:AB_2289070
- Product name
- Mouse Monoclonal CGI-16 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. ACOT9 - acyl-Coenzyme A thioesterase 2, mitochondrial
- Reactivity
- Human, Rat
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Proteomic analysis of left ventricular remodeling in an experimental model of heart failure.
Cieniewski-Bernard C, Mulder P, Henry JP, Drobecq H, Dubois E, Pottiez G, Thuillez C, Amouyel P, Richard V, Pinet F
Journal of proteome research 2008 Nov;7(11):5004-16
Journal of proteome research 2008 Nov;7(11):5004-16
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CGI-16 Antibody (4E4) [H00023597-M01] - Analysis of ACOT9 expression in transfected 293T cell line by ACATE2 monoclonal antibody (M01), clone 4E4.Lane 1: ACOT9 transfected lysate(24 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CGI-16 Antibody (4E4) [H00023597-M01] - ACATE2 monoclonal antibody (M01), clone 4E4 Analysis of ACATE2 expression in MCF-7.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: CGI-16 Antibody (4E4) [H00023597-M01] - Detection limit for recombinant GST tagged ACOT9 is approximately 3ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: CGI-16 Antibody (4E4) [H00023597-M01] - Analysis of monoclonal antibody to ACOT9 on HeLa cell. Antibody concentration 10 ug/ml.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: CGI-16 Antibody (4E4) [H00023597-M01] - Analysis of monoclonal antibody to ACOT9 on formalin-fixed paraffin-embedded human heart. Antibody concentration 3 ug/ml.