14862-1-AP

antibody from Invitrogen Antibodies

Targeting: THOC5 C22orf19, Fmip, fSAP79, KIAA0983, PK1.3

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

14862-1-AP

Provider

Invitrogen Antibodies

Product name

THOC5 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: CKDDSVLHL TFYYLMNLNI MTVKAKVTTA MELITPISAG DLLSPDSVLS CLYPGDHGKK TPNPANQYQF DKVGILTLSD YVLELGHPYL WVQKLGGLHF PKEQPQQTVI ADHSLSASHM ETTMKLLKTR VQSRLALHKQ FASLEHGIVP VTSDCQYLFP AKVVSRLVKW VTIAHEDYME LHFTKDIVDA GLAGDTNLYY MALIERGTAK LQAAVVLNPG YSSIPPIFQL CLNWKGEKTN SNDDNIRAME GEVNVCYKEL CGPWPSHQLL TNQLQRLCVL LDVYLETESH DDSVEGPKEF PQEKMCLRLF RGPSRMKPFK YNHPQGFFSH R (354-683 aa encoded by BC003615)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.21 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 14862-1-AP (THOC5 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 14862-1-AP (THOC5 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HEK-293 cells were subjected to SDS PAGE followed by western blot with 14862-1-AP ( THOC5 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of HepG2 cells using 14862-1-AP (THOC5 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 14862-1-AP (THOC5 Antibody) at dilution of 1:50 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 14862-1-AP ( THOC5 Antibody) at dilution of 1:50 (under 40x lens).

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

1X10^6 HEK-293 cells were stained with 0.2ug THOC5 antibody (14862-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1500.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-THOC5 (IP:14862-1-AP, 3ug; Detection:14862-1-AP 1:300) with HEK-293 cells lysate 2000ug.