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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Other assay [1]
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Validation data
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- Product number
- PA5-30955 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- DUT Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, Jurkat, Raji.
- Concentration
- 0.49 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of DUT using 30 µg of A431 lysate. Samples were loaded onto a 15% SDS-PAGE gel and probed with a DUT polyclonal antibody (Product # PA5-30955) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using DUT Polyclonal Antibody (Product # PA5-30955). Whole cell extract (30 µg) was separated by 15% SDS-PAGE, and the membrane was blotted with DUT Polyclonal Antibody (Product # PA5-30955) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded A549 xenograft, using DUT (Product # PA5-30955) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of DUT was performed in A431 whole cell extracts using 5 µg of DUT Polyclonal Antibody (Product # PA5-30955). Samples were transferred to a membrane and probed with DUT Polyclonal Antibody as a primary antibody and an HRP-conjugated anti-Rabbit IgG was used as a secondary antibody.
