PA5-21833

antibody from Invitrogen Antibodies

Targeting: ALOXE3 E-LOX, eLOX3

Antibody data

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Validation data

Product number: PA5-21833 - Provider product page
Provider: Invitrogen Antibodies
Product name: ALOXE3 Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Recombinant protein fragment
Description: Recommended positive controls: HepG2. Predicted reactivity: Mouse (84%), Rat (84%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Reactivity: Human
Host: Rabbit
Isotype: IgG
Vial size: 100 µL
Concentration: 1 mg/mL
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

ALOXE3 protein structure - PA5-21833 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: DsiRNA-mediated knockdown of ALOXE3 in EA.hy926 cells. ( A ): Genomic organization of ALOXE3 on chromosome 17 showing the location of three DsiRNAs designed for ALOXE3 knockdown. Blue rectangles indicate exons and red bars indicate the locations targeted by the DsiRNAs. ( B ): Bright field and fluorescent images showing transfection efficiency of TYE-563 labeled control DsiRNA. ( C ): Immunoblots with anti-ALOXE3 and anti-GAPDH antibodies in EA.hy926 cells transfected with control DsiRNA (Dsi NC) and 3 ALOXE3-specific DsiRNAs. The three bands detected by the antibody are labelled according to their approximate molecular mass based on the standards (M). ( D ): Bar graph showing the level of p100 band (only band to decrease in response to DsiRNA transfection and therefore assumed to be ALOXE3) normalized to GAPDH in DsiRNA-transfected EA.hy926 cells. Data represent mean +- SEM. N = 9, 10-15 replicates from 2 independent experiments. Data were analyzed by one-way ANOVA followed by Dunnett's multiple comparisons test. *, p

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Relative levels of ALOXE3 in confluent and non-confluent EA.hy926 endothelial cells and in confluent HUAEC endothelial cells. ( A ): Lysates of EA.hy926 cells that were either 10-20% confluent or 100% confluent were prepared in triplicate and immunoblotted for ALOXE3 and NPM1 (loading control). Representative blots are depicted in the figure. NS: non-specific bands. ( B ): Bar graph showing the relative levels in confluent and non-confluent EA.hy926 cells of ALOXE3 (100 kDa band) based on densitometry of the bands in panel A normalized to NPM1. Data represent mean +- SEM. N = 3, from 2 independent experiments. Data were analyzed by t -test (unpaired). *, p 0.05).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Effect of shear stress on ALOXE3 expression in EA.hy926 cells and HUAECs. ( A ): Brightfield images showing confluent EA.hy926 cells (top and middle panels) and HUAECs (bottom panels) incubated for 24 h under static (no shear stress) or flow (30 Dyn/cm 2 shear stress) conditions. Yellow arrows indicate the direction of flow. Middle panels show a magnified view of the region delineated in the upper panels. Scale bar: 100 mum. ( B ): Immunoblots of ALOXE3 and GAPDH (loading control) levels under static (0 Dyn/cm 2 shear stress) and flow (8, 14, 30 Dyn/cm 2 shear stress) conditions for EA.hy926 cells and 0 and 30 Dyn/cm 2 shear stress for HUAECs (passage 3). M: molecular mass markers; NS: non-specific band; SS: shear stress; FR: flow rate. ( C ): Bar graphs showing the levels of ALOXE3 protein normalized to GAPDH based on densitometry of the bands in panel B for EA.hy926 cells and HUAECs. Data are presented as mean +- SEM relative to no shear stress condition (band intensity set to 100%). N = 4 independent experiments. Data were analyzed by unpaired t -test. *, p