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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Western blot [4]
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- Product number
- PA5-27794 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Arylsulfatase B Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: HeLa, HepG2, ARSB tansfected 293T cell, mouse placenta, rat testis. Predicted reactivity: Dog (86%), Cat (89%), Bovine (90%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 6.11 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Arylsulfatase B was performed by separating 50 µg of mouse tissue extract by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a Arylsulfatase B Polyclonal Antibody (Product # PA5-27794) at a dilution of 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Arylsulfatase B was performed by separating 50 µg of rat tissue extract by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a Arylsulfatase B Polyclonal Antibody (Product # PA5-27794) at a dilution of 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of Arylsulfatase B was performed by separating 30 µg of various whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a Arylsulfatase B Polyclonal Antibody (Product # PA5-27794) at a dilution of 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of ARSB expression in transfected 293T cell line by ARSB polyclonal antibody. Lane A: Non-transfected lysate. Lane B: ARSB transfected lysate. 12% SDS PAGE. Arylsulfatase B Polyclonal Antibody (Product # PA5-27794) diluted at 1:500.
