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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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- Product number
- RQ4051 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- PER1 Antibody / Period circadian protein homolog 1
- Antibody type
- Polyclonal
- Description
- This highly specific PER1 antibody is suitable for use in Western blot/Immunohistochemistry/ELISA applications with human, mouse and rat samples.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 100 ug
- Concentration
- 0.5mg/ml if reconstituted with 0.2ml sterile DI water
- Storage
- After reconstitution, the PER1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of 1) human PANC-1, 2) human MCF7, 3) human COLO320, 4) rat heart and 5) mouse brain lysate with PER1 antibody at 0.5ug/ml. Predicted molecular weight ~136 kDa, observed here at ~200 kDa.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of 1) human A549, 2) human 293T, 3) human A431, 4) human HeLa, 5) rat brain, 6) rat PC-3, 7) mouse brain and 8) mouse HEPA1-6 cell lysate with PER1 antibody at 0.5ug/ml. Predicted molecular weight ~136 kDa, may be observed at ~200 kDa due to ubiquitination.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Immunofluorescent staining of FFPE human SiHa cells with PER1 antibody (green). HIER: steam section in pH6 citrate buffer for 20 min.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE rat brain tissue with PER1 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE human gastric cancer tissue with PER1 antibody at 1ug/ml. Required HIER: steam section in pH8 EDTA buffer for 20 min and allow to cool prior to testing.
