PA1-524

antibody from Invitrogen Antibodies

Targeting: PER1 PER, RIGUI

Western blot

Immunohistochemistry

Antibody data

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Validation data

Product number: PA1-524 - Provider product page
Provider: Invitrogen Antibodies
Product name: PER1 Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Synthetic peptide
Description: PA1-524 detects PER1 from human and mouse tissues as well as recombinant mouse PER1. PA1-524 has been sucessfully used in Western blot, immunofluoresence and immunohistochemistry procedures. By Western blot, this antibody detects an ~136 kDa protein representing overexpressed recombinant mouse PER1 protein. Immunofluorescence staining of PER1 in mouse testes with PA1-524 results in cytoplasmic staining. PA1-524 immunizing peptide corresponds to amino acid residues 39-51 from mouse PER1. PA1-524 immunizing peptide (Cat. # PEP-076) is available for use in neutralization and control experiments.
Reactivity: Human, Mouse
Host: Rabbit
Isotype: IgG
Vial size: 200 µL
Concentration: 1 mg/mL
Storage: -20° C, Avoid Freeze/Thaw Cycles

PER1 protein structure - PA1-524 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of PER1 (green) showing staining in the cytoplasm and nucleus of Hela cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a PER1 polyclonal antibody (Product # PA1-524) in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4 ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of PER1 (green) showing staining in the cytoplasm and nucleus of NIH-3T3 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a PER1 polyclonal antibody (Product # PA1-524) in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4 ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of PER1 (green) showing staining in the cytoplasm and nucleus of SH-SY5Y cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a PER1 polyclonal antibody (Product # PA1-524) in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4 ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.

Supportive validation

Supportive validation

Supportive validation

Supportive validation

Supportive validation

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 6 CK1delta is not elevated in the brains of APP-PS1 mice, although CK1 inhibition did alter PER1 expression. ( A , B ) Separate Western blots were run for tissue from each brain region. There were no differences in the basal expression of CK1delta between nontransgenic controls (nTg) and vehicle-treated APP-PS1 mice, although 30 mg/kg PF-670462 given once daily at ZT10 did increase the expression of CK1delta in the dorsal hippocampus of APP-PS1 mice compared to vehicle-treated APP-PS1. Samples were cropped between the CK1delta band and the actin bands from the same blot for each brain region. ( C , D ) At 200x magnification, there were no differences between groups in PER1 expression at ZT10, although PER1 was increased in the prefrontal cortex and anterior hypothalamus of vehicle-treated APP-PS1 mice compared to nontransgenic controls, and PF-670462 reversed this effect. Data are presented as mean +- SEM. (N = 3-5 per group; * indicates significance).