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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [3]
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- Product number
- AM05628PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AM05628PU-N, RRID:AB_1623377
- Product name
- anti VAMP-2 / Synaptobrevin-2 (N-term)
- Antibody type
- Monoclonal
- Antigen
- Recombinant Human Synaptobrevin 2 (1-89 aa) purified from E. coli.
- Reactivity
- Human, Rat
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 3E5
- Vial size
- 0.1 mg
- Concentration
- 1.0 mg/ml
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- The recombinant Synaptobrevin 1,2(each 20ng) and the extracts of Rat brain(20ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Synaptobrevin 2 (1:2,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western Blot analysis: The recombinant Synaptobrevin 1,2 (each 20ng) and the extracts of Rat brain(20ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-Human Synaptobrevin 2 (1/2,000). Proteins were visualized using a goat anti-Mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1: Synaptobrevin 1 Lane 2: Synaptobrevin 2 Lane 3. Rat brain
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Human Adrenal (formalin-fixed, paraffin-embedded) stained with VAMP2 antibody at 10 µg/ml followed by biotinylated anti-mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Human Brain (formalin-fixed, paraffin-embedded) stained with VAMP2 antibody at 10 µg/ml followed by biotinylated anti-mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Human Testis (formalin-fixed, paraffin-embedded) stained with VAMP2 antibody at 10 µg/ml followed by biotinylated anti-mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
