Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [3]
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Validation data
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- Product number
- AM05628PU-S - Provider product page
- Provider
- OriGene
- Product name
- VAMP2 (N-term) mouse monoclonal antibody, clone 3E5, Purified
- Antibody type
- Monoclonal
- Description
- VAMP2 (N-term) mouse monoclonal antibody, clone 3E5, Purified
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- VAMP2
- Isotype
- IgG
- Antibody clone number
- 3E5
- Vial size
- 50 µl
- Concentration
- 1.0 mg/ml
Submitted references Methionine Sulfoxide Reductase-B3 (MsrB3) Protein Associates with Synaptic Vesicles and its Expression Changes in the Hippocampi of Alzheimer's Disease Patients.
Adams SL, Benayoun L, Tilton K, Chavez OR, Himali JJ, Blusztajn JK, Seshadri S, Delalle I
Journal of Alzheimer's disease : JAD 2017;60(1):43-56
Journal of Alzheimer's disease : JAD 2017;60(1):43-56
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western Blot analysis: The recombinant Synaptobrevin 1,2 (each 20ng) and the extracts of Rat brain(20ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-Human Synaptobrevin 2 (1/2,000). Proteins were visualized using a goat anti-Mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1: Synaptobrevin 1 Lane 2: Synaptobrevin 2 Lane 3. Rat brain
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- The recombinant Synaptobrevin 1,2(each 20ng) and the extracts of Rat brain(20ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Synaptobrevin 2 (1:2,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Human Testis (formalin-fixed, paraffin-embedded) stained with VAMP2 antibody at 10 ug/ml followed by biotinylated anti-mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Human Brain (formalin-fixed, paraffin-embedded) stained with VAMP2 antibody at 10 ug/ml followed by biotinylated anti-mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Human Adrenal (formalin-fixed, paraffin-embedded) stained with VAMP2 antibody at 10 ug/ml followed by biotinylated anti-mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
- Validation comment
- IHC