MAB5136

antibody from R&D Systems

Targeting: VAMP2 SYB2, VAMP-2

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

MAB5136

Provider

R&D Systems

Product name

Human/Mouse VAMP-2 Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Detects human and mouse VAMP-2 in Western blots. In Western blots, no cross-reactivity with recombinant human VAMP-1, -5, -7, or -8 is observed.

Reactivity

Human, Mouse

Host

Mouse

Conjugate

Unconjugated

Antigen sequence

P63027


Isotype

IgG

Antibody clone number

541405

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Submitted references

Ca(2+)-dependent Focal Exocytosis of Golgi-derived Vesicles Helps Phagocytic Uptake in Macrophages.

Vashi N, Andrabi SB, Ghanwat S, Suar M, Kumar D
The Journal of biological chemistry 2017 Mar 31;292(13):5144-5165

Western blot

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human and Mouse VAMP-2 by Western Blot. Western blot shows lysates of Daudi human Burkitt's lymphoma cell line, Jurkat human acute T cell leukemia cell line, and mouse brain tissue. PVDF Membrane was probed with 0.1 µg/mL of Mouse Anti-Human/Mouse VAMP-2 Monoclonal Antibody (Catalog # MAB5136) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for VAMP-2 at approximately 13 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.