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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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- Product number
- PA5-25015 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GLD2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody is predicted to react with bovine based on sequence homology.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µL
- Concentration
- 0.45 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of GLD2 in MCF-7 cells. Samples were incubated with GLD2 polyclonal antibody (Product # PA5-25015) using a dilution of 1:25 followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG at a dilution of 1:400 (green). Cytoplasmic actin was counterstained with Alexa Fluor® 555 conjugated with Phalloidin (red).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of GLD2 in paraffin-embedded human cerebellum section. Samples were incubated with GLD2 polyclonal antibody (Product # PA5-25015) using a dilution of 1:25 followed by an undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of GLD2 in formalin fixed and paraffin embedded human brain tissue. Samples were incubated with GLD2 polyclonal antibody (Product # PA5-25015) followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. Clinical relevance has not been evaluated.
