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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [3]
- Immunohistochemistry [1]
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- Product number
- NBP1-32581 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-32581, RRID:AB_2161479
- Product name
- Rabbit Polyclonal PDK3 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Acidosis-induced metabolic reprogramming in tumor cells enhances the anti-proliferative activity of the PDK inhibitor dichloroacetate.
Differing roles of pyruvate dehydrogenase kinases during mouse oocyte maturation.
Pyruvate dehydrogenase kinase expression and metabolic changes following dichloroacetate exposure in anoxic human colorectal cancer cells.
Schoonjans CA, Joudiou N, Brusa D, Corbet C, Feron O, Gallez B
Cancer letters 2020 Feb 1;470:18-28
Cancer letters 2020 Feb 1;470:18-28
Differing roles of pyruvate dehydrogenase kinases during mouse oocyte maturation.
Hou X, Zhang L, Han L, Ge J, Ma R, Zhang X, Moley K, Schedl T, Wang Q
Journal of cell science 2015 Jul 1;128(13):2319-29
Journal of cell science 2015 Jul 1;128(13):2319-29
Pyruvate dehydrogenase kinase expression and metabolic changes following dichloroacetate exposure in anoxic human colorectal cancer cells.
Ho N, Coomber BL
Experimental cell research 2015 Feb 1;331(1):73-81
Experimental cell research 2015 Feb 1;331(1):73-81
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PDK3 Antibody [NBP1-32581] - Mouse tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with PDK3 antibody [N1C2] diluted at 1:500.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PDK3 Antibody [NBP1-32581] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with PDK3 antibody [N1C2] diluted at 1:1000.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PDK3 Antibody [NBP1-32581] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PDK3 antibody [N1C2] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: PDK3 Antibody [NBP1-32581] - Ca922 xenograft, using PDK3 antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
