PA5-88641

antibody from Invitrogen Antibodies

Targeting: UQCR10 HSPC051, QCR9, UCCR7.2, UCRC

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-88641

Provider

Invitrogen Antibodies

Product name

UCRC Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: MAAATLTSKL YSLLFRRTST FALTIIVGVM FFERAFDQGA DAIYDHINEG KLWKHIKHKY ENK; Positive Samples: 22RV1, H460, Mouse kidney, Rat heart; Cellular Location: Mitochondrion inner membrane

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.38 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of extracts of various cell lines, using UQCR10 Polyclonal antibody (Product # PA5-88641) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Exposure time: 30s.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of UCRC in extracts of various cell lines using UCRC Polyclonal Antibody (Product # PA5-88641) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of UCRC was performed in U2OS cells using UCRC Polyclonal Antibody (Product # PA5-88641) at a dilution of 1:100. Blue: DAPI for nuclear staining.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of UCRC in paraffin-embedded rat ovary using UCRC Polyclonal Antibody (Product # PA5-88641) at a dilution of 1:100.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of UCRC in paraffin-embedded rat heart using UCRC Polyclonal Antibody (Product # PA5-88641) at a dilution of 1:100.