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Western blot
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- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- ELISA [3]
- Immunoprecipitation [3]
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Validation data
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- Product number
- LS-C197880 - Provider product page
- Provider
- LSBio
- Product name
- CXCL4 / PF4 Antibody (clone 3F6) LS-C197880
- Antibody type
- Monoclonal
- Description
- Purified from ascites by Protein A
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 3F6
- Storage
- Store at -20°C or lower. Aliquot to avoid freeze-thaw cycles.
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Enhanced validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western Blot analysis of PF4 expression in transfected 293T cell line by PF4 monoclonal antibody (M01), clone 3F6.Lane 1: PF4 transfected lysate(10.8 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Detection limit for recombinant GST tagged PF4 is approximately 1 ng/ml as a capture antibody.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged PF4 is approximately 1 ng/ml as a capture antibody.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged PF4 is approximately 1 ng/ml as a capture antibody.
Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Immunoprecipitation of PF4 transfected lysate using anti-PF4 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PF4 rabbit polyclonal antibody.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Immunoprecipitation of PF4 transfected lysate using anti-PF4 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PF4 rabbit polyclonal antibody.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Immunoprecipitation of PF4 transfected lysate using anti-PF4 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PF4 rabbit polyclonal antibody.
