MAB595-100

antibody from R&D Systems

Targeting: PF4 CXCL4, SCYB4

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Antibody Data

Product number

MAB595-100

Provider

R&D Systems

Product name

Mouse CXCL4/PF4 Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Detects mouse CXCL4/PF4 in ELISAs and Western blots. In ELISAs, this antibody does not cross-react or interfere recombinant human (rh) CXCL/PF4, recombinant mouse (rm) CXCL9/MIG, rmGM-CSF, rmCXCL11/I-TAC, rhCXCL8/IL-8, rmIL-5, or rhCXCL10/IP-10. In Western blots, no cross-reactivity was observed with rhGCP-2, rmGCP-2, rhGRO alpha , rhGRO beta , rhGRO gamma , rhMIP-1 alpha , rmMIP-1 alpha , rmMIP-1 beta , rhMIP-1 delta , rmMIP-1 gamma , rmMIP-2, rhMIP-3 alpha , rmMIP-3 alpha , rrMIP-3 alpha , rhMIP-3 beta , or rmMIP-3 beta ,

Reactivity

Mouse

Host

Rat

Conjugate

Unconjugated

Antigen sequence

Q9Z126


Isotype

IgG

Antibody clone number

140910

Vial size

100 ug

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Submitted references

Platelet glycoprotein VI and C-type lectin-like receptor 2 deficiency accelerates wound healing by impairing vascular integrity in mice.

Wichaiyo S, Lax S, Montague SJ, Li Z, Grygielska B, Pike JA, Haining EJ, Brill A, Watson SP, Rayes J
Haematologica 2019 Aug;104(8):1648-1660

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Inhibition of profibrotic microRNA-21 affects platelets and their releasate.

Barwari T, Eminaga S, Mayr U, Lu R, Armstrong PC, Chan MV, Sahraei M, Fernández-Fuertes M, Moreau T, Barallobre-Barreiro J, Lynch M, Yin X, Schulte C, Baig F, Pechlaner R, Langley SR, Zampetaki A, Santer P, Weger M, Plasenzotti R, Schosserer M, Grillari J, Kiechl S, Willeit J, Shah AM, Ghevaert C, Warner TD, Fernández-Hernando C, Suárez Y, Mayr M
JCI insight 2018 Nov 2;3(21)

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Munc13-4 is a limiting factor in the pathway required for platelet granule release and hemostasis.

Ren Q, Wimmer C, Chicka MC, Ye S, Ren Y, Hughson FM, Whiteheart SW
Blood 2010 Aug 12;116(6):869-77

Western blot

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Mouse CXCL4/PF4 by Western Blot. Western blot shows lysate of mouse platelets. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse CXCL4/PF4 Monoclonal Antibody (Catalog # MAB595) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for CXCL4/PF4 at approximately 11 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.