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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunoprecipitation [1]
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- Product number
- H00005196-M01 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00005196-M01, RRID:AB_509366
- Product name
- PF4 monoclonal antibody (M01), clone 3F6
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a partial recombinant PF4.
- Antigen sequence
AEAEEDGDLQCLCVKTTSQVRPRHITSLEVIKAGP
HCPTAQLIATLKNGRKICLDLQAPLYKKIIKKLLE
S- Isotype
- IgG
- Antibody clone number
- 3F6
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of PF4 expression in transfected 293T cell line by PF4 monoclonal antibody (M01), clone 3F6.Lane 1: PF4 transfected lysate(10.8 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged PF4 is approximately 1ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoprecipitation of PF4 transfected lysate using anti-PF4 monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with PF4 MaxPab rabbit polyclonal antibody.
- Validation comment
- Immunoprecipitation
- Protocol
- Protocol
