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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- ANT-054-25UL - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PMAT (SLC29A4) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 25 µL
- Concentration
- 0.8 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of mouse brain lysate (lanes 1 and 3) and rat brain lysates (lanes 2 and 4): - 1-2. Anti-PMAT (SLC29A4) Antibody (#ANT-054), (1:400). 3-4. Anti-PMAT (SLC29A4) Antibody , preincubated with PMAT (SLC29A4) Blocking Peptide (BLP-NT054).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of human SH-SY5Y neuroblastoma cell line lysate (lanes 1 and 3) and human Caco2 colorectal adenocarcinoma cell line lysate (lanes 2 and 4): - 1-2. Anti-PMAT (SLC29A4) Antibody (#ANT-054), (1:400). 3-4. Anti-PMAT (SLC29A4) Antibody , preincubated with PMAT (SLC29A4) Blocking Peptide (BLP-NT054).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Expression of PMAT in rat supraoptic nucleus (SON). Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-PMAT (SLC29A4) Antibody (#ANT-054), (1:1000), followed by donkey Anti-rabbit-biotin and Streptavidin-Cy3. A. PMAT immunoreactivity (red) appears in SON neurons (arrows). B. Pre-incubation of the Antibody with PMAT (SLC29A4) Blocking Peptide (BLP-NT054), suppressed staining. Cell nuclei are stained with DAPI (blue). Abbreviations: OT = optic tract, SON = supraoptic nucleus.
