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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [2]
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- Product number
- PA5-28889 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- BTBD10 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: NT2D1, mouse testis. Predicted reactivity: Mouse (100%), Rat (100%), Xenopus laevis (98%), Chicken (99%), Rhesus Monkey (100%), Bovine (100%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.95 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using BTBD10 Polyclonal Antibody (Product # PA5-28889). Sample (30 µg of whole cell lysate). Lane A: NT2D1. 10% SDS PAGE. BTBD10 Polyclonal Antibody (Product # PA5-28889) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using BTBD10 Polyclonal Antibody (Product # PA5-28889). Sample (50 µg of whole cell lysate). Lane A: mouse testis. 10% SDS PAGE. BTBD10 Polyclonal Antibody (Product # PA5-28889) diluted at 1:500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- BTBD10 Polyclonal Antibody detects BTBD10 protein at nucleus by immunofluorescent analysis. Sample: NT2D1 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: BTBD10 protein stained by BTBD10 Polyclonal Antibody (Product # PA5-28889) diluted at 1:500. Blue: Hoechst 33342 staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- BTBD10 Polyclonal Antibody detects BTBD10 protein at nucleus by immunofluorescent analysis. Sample: NT2D1 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: BTBD10 protein stained by BTBD10 Polyclonal Antibody (Product # PA5-28889) diluted at 1:500. Blue: Hoechst 33342 staining.
