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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [2]
- Other assay [2]
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Validation data
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- Product number
- PA5-21472 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- DMC1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, HeLa, Molt-4, Raji, mouse testis. Predicted reactivity: Mouse (94%), Rat (95%), Zebrafish (83%), Japanese Medaka (84%), Bovine (98%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.9 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Meiotic gene expression initiates during larval development in the sea urchin.
Yajima M, Suglia E, Gustafson EA, Wessel GM
Developmental dynamics : an official publication of the American Association of Anatomists 2013 Feb;242(2):155-63
Developmental dynamics : an official publication of the American Association of Anatomists 2013 Feb;242(2):155-63
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of DMC1 was performed by separating 50 µg of mouse tissue extract by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a DMC1 Polyclonal Antibody (Product # PA5-21472) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using DMC1 Polyclonal Antibody (Product # PA5-21472). Sample (30 µg of whole cell lysate). Lane A: Molt-4. 10% SDS PAGE. DMC1 Polyclonal Antibody (Product # PA5-21472) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- DMC1 Polyclonal Antibody detects DMC1 protein at nucleolus on mouse testis by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. DMC1 Polyclonal Antibody (Product # PA5-21472) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded Cal27 xenograft , using DMC1 (Product # PA5-21472) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
