LS-C483027
antibody from LSBio
Targeting: ARX
CT121, EIEE1, ISSX, MRX29, MRX32, MRX33, MRX36, MRX38, MRX43, MRX54, MRX76, MRX87, MRXS1, PRTS
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [14]
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Validation data
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- Product number
- LS-C483027 - Provider product page
- Provider
- LSBio
- Product name
- ARX Antibody (aa2-50) LS-C483027
- Antibody type
- Polyclonal
- Description
- Antiserum
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Storage
- Maintain lyophilized and reconstituted antibodies at -20°C for long term storage and at 2°C to 8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze/thaw cycles.
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Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Rabbit antibody to ARX (2-50). WB on mouse brain lysate. Blocking with 1% LFDM for 30 min at RT; Primary antibody used at 1:1500 dilution incubated overnight at 4C.
Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Rabbit antibody to ARX (2-50). IHC-P on paraffin sections of mouse spinal cord. The animal was perfused using Autoperfuser at a pressure of 110 mm Hg with 300 ml 4% FA and further post fixed overnight before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered through a 0.2 micron filter. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions. Primary antibody: dilution 1:1000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Rabbit antibody to ARX (2-50). IHC-P on paraffin sections of mouse spinal cord. The animal was perfused using Autoperfuser at a pressure of 110 mm Hg with 300 ml 4% FA and further post fixed overnight before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered through a 0.2 micron filter. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions. Primary antibody: dilution 1:1000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Rabbit antibody to ARX (2-50). IHC-P on paraffin sections of mouse spinal cord. The animal was perfused using Autoperfuser at a pressure of 110 mm Hg with 300 ml 4% FA and further post fixed overnight before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered through a 0.2 micron filter. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions. Primary antibody: dilution 1:1000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Rabbit antibody to ARX (2-50). IHC-P on paraffin sections of mouse olfactory bulb. The animal was perfused using Autoperfuser at a pressure of 110 mm Hg with 300 ml 4% FA and further post fixed overnight before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered through a 0.2 micron filter. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions. Primary antibody: dilution 1:1000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Rabbit antibody to ARX (2-50). IHC-P on paraffin sections of mouse olfactory bulb. The animal was perfused using Autoperfuser at a pressure of 110 mm Hg with 300 ml 4% FA and further post fixed overnight before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered through a 0.2 micron filter. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions. Primary antibody: dilution 1:1000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Rabbit antibody to ARX (2-50). IHC-P on paraffin sections of mouse brain. The animal was perfused using Autoperfuser at a pressure of 110 mm Hg with 300 ml 4% FA and further post fixed overnight before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered through a 0.2 micron filter. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions. Primary antibody: dilution 1:1000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Rabbit antibody to ARX (2-50). IHC-P on paraffin sections of mouse spinal cord. The animal was perfused using Autoperfuser at a pressure of 110 mm Hg with 300 ml 4% FA and further post fixed overnight before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered through a 0.2 micron filter. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions. Primary antibody: dilution 1:1000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Rabbit antibody to ARX (2-50). IHC-P on paraffin sections of mouse olfactory bulb. The animal was perfused using Autoperfuser at a pressure of 110 mm Hg with 300 ml 4% FA and further post fixed overnight before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered through a 0.2 micron filter. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions. Primary antibody: dilution 1:1000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Rabbit antibody to ARX (2-50). IHC-P on paraffin sections of mouse spinal cord. The animal was perfused using Autoperfuser at a pressure of 110 mm Hg with 300 ml 4% FA and further post fixed overnight before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered through a 0.2 micron filter. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions. Primary antibody: dilution 1:1000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Rabbit antibody to ARX (2-50). IHC-P on paraffin sections of mouse spinal cord. The animal was perfused using Autoperfuser at a pressure of 110 mm Hg with 300 ml 4% FA and further post fixed overnight before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered through a 0.2 micron filter. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions. Primary antibody: dilution 1:1000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Rabbit antibody to ARX (2-50). IHC-P on paraffin sections of mouse spinal cord. The animal was perfused using Autoperfuser at a pressure of 110 mm Hg with 300 ml 4% FA and further post fixed overnight before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered through a 0.2 micron filter. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions. Primary antibody: dilution 1:1000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Rabbit antibody to ARX (2-50). IHC-P on paraffin sections of mouse olfactory bulb. The animal was perfused using Autoperfuser at a pressure of 110 mm Hg with 300 ml 4% FA and further post fixed overnight before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered through a 0.2 micron filter. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions. Primary antibody: dilution 1:1000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Rabbit antibody to ARX (2-50). IHC-P on paraffin sections of mouse olfactory bulb. The animal was perfused using Autoperfuser at a pressure of 110 mm Hg with 300 ml 4% FA and further post fixed overnight before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered through a 0.2 micron filter. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions. Primary antibody: dilution 1:1000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Rabbit antibody to ARX (2-50). IHC-P on paraffin sections of mouse brain. The animal was perfused using Autoperfuser at a pressure of 110 mm Hg with 300 ml 4% FA and further post fixed overnight before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered through a 0.2 micron filter. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions. Primary antibody: dilution 1:1000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.