PA5-46969

antibody from Invitrogen Antibodies

Targeting: EREG ER

Western blot (Recommended by provider)

ELISA (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Blocking/Neutralizing (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-46969

Provider

Invitrogen Antibodies

Product name

Epiregulin Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

In direct ELISAs and Western blots, approximately 50% cross-reactivity with recombinant mouse Epiregulin is observed. Reconstitute at 0.2 mg/mL in sterile PBS. Endoxin level is

Reactivity

Human

Host

Goat

Isotype

IgG

Vial size

100 µg

Concentration

0.2 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

References

Submitted references

The homeostatic malfunction of a novel feedback pathway formed by lncRNA021545, miR-330-3p and epiregulin contributes in hepatocarcinoma progression via mediating epithelial-mesenchymal transition.

Yang S, Zhang Y, Guo C, Liu R, Elkharti M, Ge Z, Liu Q, Liu S, Sun MZ
American journal of cancer research 2022;12(6):2492-2525

ELISA

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

ELISA of Epiregulin in recombinant Human Epiregulin protein. Samples were incubated in Epiregulin polyclonal antibody (Product # PA5-46969). Protein was serially diluted 2-fold. Antibody was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP followed by Substrate Solution and stopping the enzymatic reaction with Stop Solution.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Neutralization of Epiregulin in Balb/3T3 mouse embryonic fibroblast cell line. Samples were incubated in Epiregulin polyclonal antibody (Product # PA5-46969). Recombinant Human Epiregulin stimulates proliferation in the Balb/3T3 mouse embryonic fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human Epiregulin (5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human Epiregulin Antigen Affinity-purified Polyclonal Antibody. The ND50 is typically 0.3-1.5 µg/mL.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Neutralization antibody testing demonstrates the specificity of an antibody through a correlation between antibody binding and the activity of the target. Neutralization of Epiregulin is shown by the decrease in mean RFU (relative fluorescent units - measure of Epiregulin induced proliferation of Balb/3T3 cells) with increasing concentrations of Epiregulin Polyclonal Antibody (PA5-46969).