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Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- ELISA [1]
- Immunohistochemistry [1]
- Other assay [2]
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- Product number
- PA5-46969 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Epiregulin Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- In direct ELISAs and Western blots, approximately 50% cross-reactivity with recombinant mouse Epiregulin is observed. Reconstitute at 0.2 mg/mL in sterile PBS. Endoxin level is
- Reactivity
- Human
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.2 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references The homeostatic malfunction of a novel feedback pathway formed by lncRNA021545, miR-330-3p and epiregulin contributes in hepatocarcinoma progression via mediating epithelial-mesenchymal transition.
Yang S, Zhang Y, Guo C, Liu R, Elkharti M, Ge Z, Liu Q, Liu S, Sun MZ
American journal of cancer research 2022;12(6):2492-2525
American journal of cancer research 2022;12(6):2492-2525
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ELISA of Epiregulin in recombinant Human Epiregulin protein. Samples were incubated in Epiregulin polyclonal antibody (Product # PA5-46969). Protein was serially diluted 2-fold. Antibody was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP followed by Substrate Solution and stopping the enzymatic reaction with Stop Solution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Epiregulin in immersion fixed paraffin-embedded sections of human skin. Samples were incubated in Epiregulin polyclonal antibody (Product # PA5-46969) using a dilution of 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown) and counterstained with hematoxylin (blue). Specific staining was localized to keratinocytes.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Neutralization of Epiregulin in Balb/3T3 mouse embryonic fibroblast cell line. Samples were incubated in Epiregulin polyclonal antibody (Product # PA5-46969). Recombinant Human Epiregulin stimulates proliferation in the Balb/3T3 mouse embryonic fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human Epiregulin (5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human Epiregulin Antigen Affinity-purified Polyclonal Antibody. The ND50 is typically 0.3-1.5 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Neutralization antibody testing demonstrates the specificity of an antibody through a correlation between antibody binding and the activity of the target. Neutralization of Epiregulin is shown by the decrease in mean RFU (relative fluorescent units - measure of Epiregulin induced proliferation of Balb/3T3 cells) with increasing concentrations of Epiregulin Polyclonal Antibody (PA5-46969).
