PA5-117910

antibody from Invitrogen Antibodies

Targeting: DHTKD1 CMT2Q, DKFZP762M115, KIAA1630, MGC3090

Western blot (Data presented on provider website)

Immunoprecipitation (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-117910

Provider

Invitrogen Antibodies

Product name

DHTKD1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation of DHTKD1 in Lane A: 0.5 mg Hela Whole Cell lysate, Lane B: 0.5 mg A549 Whole Cell Lysate. Samples were treated with 15 μl of 50 % Protein G agarose, incubated with polyclonal antibody (Product # PA5-117910) with a dilution of 1:100 , followed by Dylight 800-labeled antibody to rabbit IgG (H+L) using a dilution of 1:5,000. Assay was performed under reducing conditions. Predicted band size: 101 kDa, Observed band size: 101 kDa .

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

DHTKD1 Immunoprecipitation using: Lane A: 0.5 mg Hela Whole Cell Lysate, Lane B: 0.5 mg A549 Whole Cell Lysate 4 µL with DHTKD1 Polyclonal Antibody (Product # PA5-117910) and 15 µL of 50 % Protein G agarose. Primary antibody: DHTKD1 Polyclonal Antibody, at 1:100 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 101 kDa. Observed band size: 101 kDa.