PA5-24208

antibody from Invitrogen Antibodies

Targeting: DHTKD1 CMT2Q, DKFZP762M115, KIAA1630, MGC3090

Western blot (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-24208

Provider

Invitrogen Antibodies

Product name

DHTKD1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

This antibody is predicted to react with rat based on sequence homology.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

400 µL

Concentration

0.5 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Synthetic analogues of 2-oxo acids discriminate metabolic contribution of the 2-oxoglutarate and 2-oxoadipate dehydrogenases in mammalian cells and tissues.

Artiukhov AV, Grabarska A, Gumbarewicz E, Aleshin VA, Kähne T, Obata T, Kazantsev AV, Lukashev NV, Stepulak A, Fernie AR, Bunik VI
Scientific reports 2020 Feb 5;10(1):1886

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Dual mode action of mangiferin in mouse liver under high fat diet.

Lim J, Liu Z, Apontes P, Feng D, Pessin JE, Sauve AA, Angeletti RH, Chi Y
PloS one 2014;9(3):e90137

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 6 MGF induces Dhtkd1 and Cox6b, and suppresses Acac and Scd1 in hepatic tissues and cells. (A) Western blots of Dhtkd1, Cox6b1, Acac and Scd1 in proteins extracted from liver tissues from C57BL6/J mice fed with HFD +- MGF for 18 weeks. (B) Quantification of protein expression in (A). Liver tissues were from 2 groups (HFD +- MGF), 3 mice per group. Values are average +- SEM (n = 3). (C) Western blots of Dhtkd1, Cox6b1, Acac and Scd1 in proteins extracted from mouse primary hepatocytes and treated with or without 200 uM MGF for 24 hours. (D) Quantification of protein expression in (C). Values are average +- SEM (n = 3). *p

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 2 Levels of the proteins, mRNA and activities of OADHC and OGDHC in different rat tissues. ( A ) Comparison of DHTKD1 expression, determined by Western blot (WB) in our experiments, and extracted from transcriptomics and proteomics databases. The tissue homogenates (n = 3 for each tissue) reacted with anti-DHTKD1 antibodies (Invitrogen). Pink bars correspond to the intensities of bands in 100-110 kDa area (Fig. S1A), which were normalized to the total protein in the lane, measured by 2,2,2-trichloroethanol staining (Fig. S1B). The data extraction from the gene expression databases is described in Materials and Methods. ( B ) Mass-spectrometric detection of the DHTKD1, OGDH, and DLST proteins in mitochondria isolated from the rat liver (n = 6) or cerebral cortex (n = 5). Numbers of unique peptides, detected after in-gel trypsin digestion, are presented. (C,D) Specific activities of the rat tissue homogenates in catalysing the OADH ( C ) and OGDH reactions ( D ), assayed before (grey bars) or after solubilization of membranous compartments by sonication and detergents (black bars). ( E ) Relative levels of mRNAs for the multienzyme complex components DHTKD1 , OGDH , OGDHL , DLST and DLD in the rat tissues. The transcriptomic data are extracted from >=18 independent series of experiments for each tissue. ( F ) Relative levels of the DHTKD1, OGDH, OGDHL, DLST and DLD proteins in the mouse tissues. Except for spinal cord (1 experiment), the protein abundances were extracted