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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- MAB34691 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Mouse Wnt-10a Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects mouse and human Wnt-10a in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant mouse Wnt-1, -2, -2b, -3a, -4, -5a, -5b, recombinant human (rh) Wnt-6 or rhWnt-7a is observed.
- Reactivity
- Human, Mouse
- Host
- Rat
- Conjugate
- Unconjugated
- Antigen sequence
P70701- Isotype
- IgG
- Antibody clone number
- 342811
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Detection of Human and Mouse Wnt-10a by Western Blot. Western blot shows lysates of Raji human Burkitt's lymphoma cell line and NIH-3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse Wnt-10a Monoclonal Antibody (Catalog # MAB34691) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Wnt-10a at approximately 46 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
