NBP2-04062

antibody from Novus Biologicals

Targeting: SLAIN1 C13orf32, FLJ30046

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Antibody Data

Product number

NBP2-04062

Provider

Novus Biologicals

Product name

Rabbit Polyclonal SLAIN1 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 ul

Concentration

1.0 mg/ml

Storage

Store at 4C. Do not freeze.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: SLAIN1 Antibody [NBP2-04062] - Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from 293T cells. Antibodies: Affinity purified rabbit anti-SLAIN1 antibody NBP2-04010 used for IP at 6 ug/mg lysate. SLAIN1 was also immunoprecipitated by rabbit anti-SLAIN1 antibody NBP2-04062, which recognizes a downstream epitope. For blotting immunoprecipitated SLAIN1, NBP2-04062 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: SLAIN1 Antibody [NBP2-04062] - Detection of human SLAIN1 by western blot and immunoprecipitation. Samples: Whole cell lysate from HEK293T(15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), Jurkat (J; 50 ug) and HeLa (H; 50 ug) cells. Antibodies: Affinity purified rabbit anti-SLAIN1 antibody NBP2-04062 used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. SLAIN1 was also immunoprecipitated by another rabbit anti-SLAIN1 antibody, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).