Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- F52556 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- DHFR Antibody
- Antibody type
- Polyclonal
- Antigen
- A portion of amino acids 1-50 from human Dihydrofolate reductase was used as the immunogen for this DHFR antibody.
- Description
- Antigen affinity purified antibody
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 80 µl, 400 µl
- Storage
- Aliquot the DHFR antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot analysis of lysate from 1) 293T, 2) HeLa, 3) Jurkat, 4) Y79 cell line , 5) mouse spleen and 6) rat kidney tissue lysate using DHFR antibody at 1:1000. Predicted molecular weight ~22 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC analysis of FFPE human liver section using DHFR antibody; Ab was diluted at 1:25.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human brain section using DHFR antibody; Ab was diluted at 1:25 dilution.