MA5-38337

antibody from Invitrogen Antibodies

Targeting: DHFR

Western blot (Data presented on provider website)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-38337

Provider

Invitrogen Antibodies

Product name

DHFR Recombinant Rabbit Monoclonal Antibody (9B2)

Antibody type

Monoclonal

Antigen

Synthetic peptide

Description

This antibody has been tested in direct-ELISA

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Antibody clone number

9B2

Vial size

100 µL

Concentration

0.4 mg/mL

Storage

-20°C or -80°C if preferred

Immunoprecipitation

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitating DHFR in Hela whole cell lysate. Lane 1: Rabbit control IgG Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1:2,000). Lane 2: DHFR Monoclonal Antibody (Product # MA5-38337) (2 µg) + Hela whole cell lysate (500 µg). Lane 3: Hela whole cell lysate (10 µg).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Paraffin) analysis of DHFR in paraffin-embedded human breast cancer using DHFR Monoclonal Antibody (Product # MA5-38337) diluted at 1:100. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Paraffin) analysis of DHFR in paraffin-embedded human liver cancer using DHFR Monoclonal Antibody (Product # MA5-38337) diluted at 1:100. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitating DHFR in Hela whole cell lysate. Lane 1: Rabbit control IgG Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1:2,000). Lane 2: DHFR Monoclonal Antibody (Product # MA5-38337) (2 µg) + Hela whole cell lysate (500 µg). Lane 3: Hela whole cell lysate (10 µg).