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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [2]
- Other assay [1]
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- Product number
- MA5-38337 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- DHFR Recombinant Rabbit Monoclonal Antibody (9B2)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- This antibody has been tested in direct-ELISA
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- 9B2
- Vial size
- 100 µL
- Concentration
- 0.4 mg/mL
- Storage
- -20°C or -80°C if preferred
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of DHFR using DHFR Monoclonal Antibody (Product # MA5-38337) at 1:2,000. Positive WB detected in: Hela whole cell lysate, Jurkat whole cell lysate, K562 whole cell lysate, Raji whole cell lysate, 293T whole cell lysate, Rat heart tissue, Mouse kidney tissue, Mouse liver tissue. Secondary: Goat polyclonal to rabbit IgG at 1:50,000 dilution. Predicted band size: 22, 16 kDa. Observed band size: 22 kDa
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of DHFR in paraffin-embedded human breast cancer using DHFR Monoclonal Antibody (Product # MA5-38337) diluted at 1:100. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of DHFR in paraffin-embedded human liver cancer using DHFR Monoclonal Antibody (Product # MA5-38337) diluted at 1:100. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitating DHFR in Hela whole cell lysate. Lane 1: Rabbit control IgG Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1:2,000). Lane 2: DHFR Monoclonal Antibody (Product # MA5-38337) (2 µg) + Hela whole cell lysate (500 µg). Lane 3: Hela whole cell lysate (10 µg).
