Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- AF7934 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Dihydrofolate Reductase/DHFR Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human Dihydrofolate Reductase/DHFR in direct ELISAs and Western blots.
- Reactivity
- Human
- Host
- Sheep
- Conjugate
- Unconjugated
- Antigen sequence
P00374
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
No comments: Submit comment
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human Dihydrofolate Reductase/DHFR by Western Blot. Western blot shows lysates of COLO 205 human colorectal adenocarcinoma cell line, HEK293T human embryonic kidney cell line, and Raji human Burkitt's lymphoma cell line. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human Dihydrofolate Reductase/DHFR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7934) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Dihydrofolate Reductase/DHFR at approximately 21 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Dihydrofolate Reductase/DHFR in MCF-7 Human Cell Line. Dihydrofolate Reductase/ DHFR was detected in immersion fixed MCF-7 human breast cancer cell line using Sheep Anti-Human Dihydrofolate Reductase/ DHFR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7934) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the Northern-Lights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counter-stained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.