Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [5]
- Immunohistochemistry [5]
- Other assay [2]
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Validation data
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- Product number
- 12362-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- DNAJC1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.14 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
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- Experimental details
- NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 12362-1-AP (DNAJC1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
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- Invitrogen Antibodies (provider)
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- Experimental details
- A549 cells were subjected to SDS PAGE followed by western blot with 12362-1-AP (DNAJC1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
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- Experimental details
- Raji cells were subjected to SDS PAGE followed by western blot with 12362-1-AP (DNAJC1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
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- Invitrogen Antibodies (provider)
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- Experimental details
- MCF-7 cells were subjected to SDS PAGE followed by western blot with 12362-1-AP (DNAJC1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
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- Invitrogen Antibodies (provider)
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- Experimental details
- C6 cells were subjected to SDS PAGE followed by western blot with 12362-1-AP (DNAJC1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
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- Experimental details
- Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 12362-1-AP (DNAJC1 antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 12362-1-AP (DNAJC1 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using 12362-1-AP (DNAJC1 antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using 12362-1-AP (DNAJC1 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human colon cancer using 12362-1-AP (DNAJC1 antibody) at dilution of 1:50 (under 10x lens).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-DNAJC1 (IP:12362-1-AP, 3ug; Detection:12362-1-AP 1:300) with Raji cells lysate 2000ug.
- Submitted by
- Invitrogen Antibodies (provider)
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- Experimental details
- IP result of anti-DNAJC1 (IP:12362-1-AP, 4 ug; Detection:12362-1-AP 1:1000) with Raji cells lysate 3200 ug.