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Western blot
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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [2]
- Other assay [1]
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- Product number
- PA5-96579 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NAA20 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: PGGELMGYIM GKAEGSVARE EWHGHVTALS VAPEFRRLGL AAKLMELLEE ISERKGGFFV DLFVRVSNQV AVNMYKQLGY SVYRTVIEYY SASNGEPDED AYDMRKALSR DTEKKSIIPL PHPVRPEDIE; Positive Samples: HeLa, Mouse brain, Mouse kidney, Rat brain; Cellular Location: Cytoplasm, Nucleus
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.31 mg/mL
- Storage
- -20°C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of NAA20 was performed in A-549 cells using NAA20 Polyclonal Antibody (Product # PA5-96579).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of NAA20 in 200 μg extracts of MCF7 cells. Samples were precipitated with 1 μg NAA20 Polyclonal antibody (Product # PA5-96579). Western blot was performed from the immunoprecipitate using NAA20 Polyclonal antibody (Product # PA5-96579) at a dilution of 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of NAA20 in paraffin-embedded human oophoroma. Samples were incubated with NAA20 Polyclonal antibody (Product # PA5-96579) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of NAA20 in paraffin-embedded rat kidney. Samples were incubated with NAA20 Polyclonal antibody (Product # PA5-96579) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation analysis of NAA20 was performed in 200 µg extracts of MCF7 cells using NAA20 Polyclonal Antibody (Product # PA5-96579). Western blot was performed from the immunoprecipitate using NAA20 Polyclonal Antibody.
