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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [3]
- Other assay [1]
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- Product number
- PA5-96579 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NAA20 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: PGGELMGYIM GKAEGSVARE EWHGHVTALS VAPEFRRLGL AAKLMELLEE ISERKGGFFV DLFVRVSNQV AVNMYKQLGY SVYRTVIEYY SASNGEPDED AYDMRKALSR DTEKKSIIPL PHPVRPEDIE; Positive Samples: HeLa, Mouse brain, Mouse kidney, Rat brain; Cellular Location: Cytoplasm, Nucleus
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.31 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of NAA20 in extracts of various cell lines using NAA20 Polyclonal Antibody (Product # PA5-96579) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of NAA20 was performed in A-549 cells using NAA20 Polyclonal Antibody (Product # PA5-96579).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of NAA20 in paraffin-embedded human colon carcinoma using NAA20 Polyclonal Antibody (Product # PA5-96579) at a dilution of 1:100.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of NAA20 in paraffin-embedded human oophoroma using NAA20 Polyclonal Antibody (Product # PA5-96579) at a dilution of 1:100.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of NAA20 in paraffin-embedded human esophageal cancer using NAA20 Polyclonal Antibody (Product # PA5-96579) at a dilution of 1:100.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation analysis of NAA20 was performed in 200 µg extracts of MCF7 cells using NAA20 Polyclonal Antibody (Product # PA5-96579). Western blot was performed from the immunoprecipitate using NAA20 Polyclonal Antibody.
