711223

antibody from Invitrogen Antibodies

Targeting: DSP DPI, DPII, KPPS2, PPKS2

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

711223

Provider

Invitrogen Antibodies

Product name

Desmoplakin Recombinant Polyclonal Antibody (14HCLC)

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human

Host

Rabbit

Isotype

IgG

Antibody clone number

14HCLC

Vial size

100 µg

Concentration

0.5 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis was performed on fixed and permeabilized A-431 cells for detection of endogenous Desmoplakin /DSP using Anti-Desmoplakin /DSP Recombinant Rabbit Polyclonal Antibody (Product # 711223, 2 µg/mL) and labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representative cells that were stained for detection and localization of Desmoplakin /DSP protein (green), Panel b) is stained for nuclei (blue) using SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). Panel c) represents cytoskeletal F-actin staining using Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d) is a composite image of Panels a, b and c clearly demonstrating localization of Desmoplakin /DSP in the desmosomes at the cell junctions. Panel e) represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.