Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunocytochemistry [1]
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Validation data
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- Product number
- PAB0732 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB0732, RRID:AB_1112895
- Product name
- ATG10 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of ATG10.
- Storage
- Store at 4°C on dry atmosphere.After reconstitution with deionized water, store at -20°C or lower.Aliquot to avoid repeated freezing and thawing.
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Supportive validation
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- Abnova Corporation (provider)
- Main image
- Experimental details
- Confocal microscopy on immunofluorescently detected ATG10 in cytospin-isolated human white blood cells using rabbit ATG10 polyclonal antibody (Cat # PAB0732) at a dilution of 1 : 100, incubated for 1 hr at room temperature. The cells stained for ATG10 appear in red. The cells were then counter stained with Hoechst Dye (blue colour) . Here, the merged picture is presented. More information on the staining : Fixation : In acetone for 90 seconds. Antibody dilutions : Primary antibody was diluted 1 : 100 and incubated for 1 hr, washed then 3x, and the secondary (Goat anti Rb 1 : 100 alexa 546 conjugate) was incubated for 30 minutes. Magnification 80X.
- Validation comment
- Immunofluorescence